Pyrogen Testing

A

Seminar

On

pyrectic Testing

Presented by: Luhar Shailesh V.
M.Pharm-I
Dept. of Quality Assurance,
Maliba pharmacy college,
Bardoli.

Content:

? Introduction

? compartmentalization of Endotoxins

? Chemical nature and structure of the Endotoxins.





? Properties of Bacterial Endotoxins

o biological Properties

o Physical Properties

? Pyrogen Testing

o Rabbit see (sham test)

o LaL Test

? Depyrogenation Techniques

o Physically removal of Pyrogens

o deactivation of Pyrogens

Introduction

? A Pyrogen is defined as a febrility producing agent

? Pyrogens argon products of metabolism of micro organisms. Most bacterium ,viruses & many moulds have been reported as pyrogen producing.

? Pyrogen are substances that initiate febrile reactions when sufficient numbers enters the circulative system, therefore, the history of intravenous therapy and the history of pyrogen research are closely related.

? The gram negative bacteria produce the virtually potent pyrogenic substances as endotoxins.

Nature of Pyrogens:

? The term pyrogen is frequently used to describe the pyrogen most real for the pharmaceutical industry,

? Endotoxins are part of the outer membrane of the cellular telephone wall of Gram-negative bacteria. Endotoxin is invariably associated with Gram-negative bacteria whether the organisms are pathogenic or not. Although the term endotoxin is occasionally used to identify to any cell-associated bacterial toxin, in bacteriology it is properly reserved to summon to the lipopolysaccharide complex associated with the outer membrane of...If you want to get a full essay, order it on our website: Orderessay



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